A genetically encoded aldehyde for rapid protein labelling.
نویسندگان
چکیده
Using a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli. This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.
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عنوان ژورنال:
- Chemical communications
دوره 50 56 شماره
صفحات -
تاریخ انتشار 2014